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目的研究不同喂养方式对极低出生体重儿喂养状况和肠道菌群的影响。方法选择60例符合条件的住院早产儿并随机分为两组,分别为母乳喂养组(母乳组)及早产儿配方奶喂养组(配方奶组),在生后记录喂养状况,并于3、14及28天时分别留取患儿大便标本,采用实时荧光定量PCR技术检测标本中的肠道乳酸杆菌及双歧杆菌。结果母乳组喂养不耐受的发生率为5例(16.7%),配方奶组为13例(43.3%),两组比较差异有统计学意义(P<0.05)。母乳组在14天时肠道乳酸杆菌和双歧杆菌的数量分别为(8.62±1.35)cfu/g、(9.10±1.33)cfu/g,配方奶组分别为(7.32±0.80)cfu/g、(7.62±1.22)cfu/g,两组比较差异均有统计学意义(P<0.05)。母乳组在28天时肠道乳酸杆菌和双歧杆菌的数量分别为(9.43±0.26)cfu/g、(9.97±0.54)cfu/g,配方奶组分别为(8.31±0.58)cfu/g、(8.54±0.53)cfu/g,两组比较差异均有统计学意义(P<0.05)。结论喂养方式对极低出生体重早产儿喂养及肠道菌群的形成有明显影响。母乳喂养组患儿肠道的双歧杆菌和乳酸杆菌数量高于早产儿配方奶喂养组,同时喂养不耐受发生率低于早产儿配方奶喂养组。
Abstract：Objective To study the impact of different feeding patterns on feeding and intestinal flora in very low birth weight infants. Methods 60 preterm infants hospitalized were randomly divided into breast-feeding group and preterm-formula-feeding group.Stool specimens was sustained in three days after birth,2 weeks and 4 weeks of age,respectively while feeding symptoms were recorded,real-time PCR technology was used to measure intestinal specimens Lactobacillus and Bifidobacterium. Results The incidence of feeding intolerance in breast-feeding group was 16. 7%( 5 patients),and in formula-feeding group was 43. 3%( 13patients),the difference was statistically significant( P < 0. 05). The number of intestinal lactobacilli and bifidobacteria at 2 weeks in breast-feeding group were( 8. 62 ± 1. 35) cfu/g and( 9. 10 ± 1. 33) cfu/g,and in formula-feeding group were( 7. 32 ± 0. 80) cfu/g and( 7. 62 ± 1. 22) cfu/g,the difference was statistically significant( P < 0. 05). The number of intestinal lactobacilli and bifidobacteria at 4 weeks of age in breast-feeding group were( 9. 43 ± 0. 26) cfu/g and( 9. 97 ± 0. 54) cfu/g,and in formula-feeding group was( 8. 31 ± 0. 58) cfu/g and( 8. 54 ± 0. 53) cfu/g,the difference was statistically significant( P < 0. 05). Conclusion Feeding mode is an important factor for feeding and intestinal microbiota in very low birth weight infants. The number of intestinal bifidobacteria and lactobacilli in breast-feeding group were higher than those in preterm-formula-feeding group,while the incidence of feeding intolerance was significantly lower in breast-feeding group.
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基本信息:
DOI：10.19757/j.cnki.issn1674-7763.2017.04.013
中图分类号:R722.6
引用信息:
[1]吕红艳,王秋丽,杨李红等.不同喂养方式对极低出生体重儿喂养及肠道菌群的影响[J].中国妇幼卫生杂志,2017,8(04):56-59.DOI:10.19757/j.cnki.issn1674-7763.2017.04.013.
基金信息:
中国疾病预防控制中心妇幼保健中心合生元母婴营养与健康研究项目(2015FYH022)