目的了解贵州省清镇市围孕期女性血清同型半胱氨酸(homocysteine,Hcy)水平及影响因素。方法选择2019年3-6月在清镇市妇幼保健院就诊的健康围孕期女性1421人,根据知情同意原则,采用循环酶法检测血清Hcy浓度,采用荧光定量PCR法检测5,10-亚甲基四氢叶酸还原酶(MTHFR) C677T基因位点多态性。结果①<30岁和≥30岁年龄组的Hcy水平分别为(6.20±2.30)、(5.94±2.13)μmol/L,差异有统计学意义(P <0.05)。②孕前、早孕期、中孕期、晚孕期、产后的血清Hcy水平分别为(7.14±1.56)、(6.17±2.19)、(5.72±2.28)、(6.93±2.93)、(7.10±2.39)μmol/L,差异有统计学意义(P <0.05)。③根据MTHFR C677T基因位点多态性,CC、CT、TT基因型女性的围孕期血清Hcy水平分别为(7.23±2.19)、(7.60±2.20)、(9.02±2.53)μmol/L,差异有统计学意义(P <0.05)。结论围孕期女性血清Hcy水平与年龄、围孕期不同阶段以及MTHFR C677T基因多态性有关,监测Hcy水平对围孕期保健有重要的指导意义。

Objective To understand the levels of serum homocysteine( Hcy) and its influencing factors in perinatal women in Qingzhen city,Guizhou province. Methods A total of 1421 healthy perinatal women were selected from the Maternal and Child Health Hospital of Qingzhen city from March to June 2019. According to the principle of informed consent,the serum Hcy concentration was detected by circulating enzyme method,and 5,10-was detected by real-time PCR. Methylenetetrahydrofolate reductase( MTHFR) C677 T locus polymorphism. Results ①The Hcy level in the < 30 years old and ≥30 years old age groups were( 6. 20 ± 2. 30) and( 5. 94 ± 2. 13) μmol/L,respectively,and the difference was statistically significant between the two age groups( P < 0. 05) . ②The serum Hcy levels before pregnancy,first trimester,second trimester,third trimester and postpartum were( 7. 14 ±1. 56),( 6. 17 ± 2. 19),( 5. 72 ± 2. 28),( 6. 93 ± 2. 93) and( 7. 10 ± 2. 39) μmol/L,respectively,and the difference was statistically significant between different periods( P < 0. 05) . ③According to the MTHFR C677 T locus polymorphism,the serum Hcy levels of women with CC,CT and TT genotypes were( 7. 23 ± 2. 19),( 7. 60 ± 2. 20) and( 9. 02 ± 2. 53) μmol/L,respectively. The difference was statistically significant( P < 0. 05). Conclusion Serum Hcy levels in perinatal women are related to age,different periods of pregnancy and MTHFR C677 T gene polymorphism. Monitoring Hcy level has important guiding significance for perinatal health care.